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CRIMIC CR70299

Gene Gsc

CG2851

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:583540..594685	21E2	FBti0217686	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCCTCTGCCACTTCCGTTTC	CCGAGCTGAATTCAGTGTGCAT

Images

Construct Design Sequences

sgRNA sequence	AACCCATTCCTCCTGCTTCGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTCTAATTAAACTTTTTCCTCCCACCCAGTTCGCAGTGAGCTCTCGCCCCGAGTTTCCCCAGTATTTATTCAGCAAATAATCAAAAAACAGACGGACAAATAAACACATAGGGGTTGCAAATTCACCATTGCACTCGGATTGGGCAAATAGAGAATCGCTGGGAGAACGCAGAGAAAGAGAGAAAGACTCCGCCCTCGA	AGCAGGAGGAATGGGTTTTTGGGGTAGCTTTGGAGGCTAATGACAAGGCAGATGGGTTCACTGTCCACGAGGGTGATTACATAAGGGATTCCCGCTCCTCCTTCTCCCCCACATCTCGTCGCCAGAATGATAGCTTCCCTTTGCGAGGCGGAGATATCACCCTGATGATGTTTAACCCCTAAGTCACTTCACAGCCGCCT

 

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