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CRIMIC CR70303

Gene Crtc

CG6064

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:17659818..17669234	74E4-74E5	FBti0220985	95029

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCAACAGATTGTCGACTACACC	GGATCAAACAAACGAACGGAGACG

Images

Construct Design Sequences

sgRNA sequence	CGCAGACACAATCTCCACAATGG
sgRNA2 sequence	ATAACGAGATAACAATATTGAGG

 

left_200bp	right_200bp
GTATCTAACAAATGCATTCCAACGTGGCCAAATAAAAATGAATAATGAAACATTTGCTGCTAAAAATTATCATATAAATGTTGGTTACCTCTGTAGGCAAAAGCAAAACGATAGTAAAAACCCCAAAGCATCTTATCCCTTTGCAATATAATAATGATGATCTTTTTCTAAACAGCTTCCTACCGCAGACACAATCTCCA	TATTGTTATCTCGTTATCGTCCGTGCTTTCGTAGCTAGCTCCTACAACAAAAATACAAAAAAAAAAAAATTAACAAATTGTTAACCCTTAAGTGTACTTGAGTGTCTTTTTACACCCTCTTTCCTCAAAAATCCCTAGCACCCTGAAAAAGCGAAACATATTTTACAGCGAGTCTCAGATTTTACTTAACTACGCACATA

 

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