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CRIMIC CR70305

Gene SecS

CG1427

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:5601433..5603185	83B4	FBti0220986	95030

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGCTTCTTCTGGATGACTTG	CACAGCAGCGATATCAAAGTGCCA

Images

Construct Design Sequences

sgRNA sequence	CAATGTCTATCGCCGCTTTCAGG
sgRNA2 sequence	ATTTGATTGTTGTTTCTAGTAGG

 

left_200bp	right_200bp
CAACAAATTAAGTTAAGTCCATTTTAGTAGCGTTGCGAGGAGTTTATTTTCGAAAATACGCCTTTGTATATATTATATATTTTTCCTCCACTTTAAACAAAATCGAAAGGTGTCCATTTGTCAGCTATTTAAACAATATGGCATCGCCGCCTTACTTGCTCAGTAAGAAAATTTAACTGTTTTTTTTTTGCGCACCTGAA	AGAAACAACAATCAAATAAAAAACCTATTTTTTAATATTAATGATAAACTTTCACTTTTTTAGCTGATTTTTATAAGCACAATTTTGTTAGGAATGTACAGCGACTTTTATTTGTTTAGACAATTTGGCAGTGCATGCAGTAACGATATCCCACTAAAGAGACCTACATATACATATATATATTTTCCTAAGTCTTAGCT

 

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