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CRIMIC CR70306

Gene Ote

CG5581

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:18275607..18277177	55C2	FBti0220987

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGTGCCTTTGAGATGTCCATG	GATTGCAAGGCATTGCAAGGATCG

Images

Construct Design Sequences

sgRNA sequence	GGTGTGTGCTAAAATAGTGATGG
sgRNA2 sequence	AAATAAGAACAATGATGCGCCGG

 

left_200bp	right_200bp
AACGTAAATAACGATATAATAATATAGCATAGATGTTTTATTACGTTTGCACGATTTTGCGATGTGTGCGATGATTAGTATGAAACATCATTCCTTGTGTTCCCTTTTTTTAAAACGCCAAAACTGTTACAAGTCAAGCCCACTTCAGTGTTGTATATCCCCTAAAACAGAATCATCGATAGGTAATACTGCTGCCATCA	CATCATTGTTCTTATTTCGAATTTGTAATCTAGTTTGTATACCATGTTGGATATGTTTTGTAAACAAGACTGCCGCCGCGGTCTTTGTATGAATCTTGTTTTGATGTAACTTGAGCTTGAGTTTGACTAGCATTTAAGCTGCGTGTTATCCGTGTTCAAATAAAGCCCACTTGCAGGGAATCTCCACAGTTGAACACTTG

 

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