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CRIMIC CR70309

Gene Rab1

CG3320

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:21266029..21269060	93D2	FBti0220954	95033

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TTTGCCCTCGTTGGCAACTCCAAA	GATTTCTAACTGATTGTCATCCAACTTA

Images

Construct Design Sequences

sgRNA sequence	ATTACGTAGCACTGCGCTGGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AACCATAACCAAAACTGTGTGTTTCAGCTGGATTTCAATGTAGTGCCCACGGGCGAGGCGATGAAAAGCGCGTTCGAAACTCTCATCGGAATCTGCATATGTATAAAGTCCACACATATATACAGCGAGATCCCAGTTGACGGGCCAAATCGCTAGTCGGGCGGATGAGTCACCTGCGTGGAGAGCCTTTCCCACCCCCA	GCGCAGTGCTACGTAATCAAAGGCCATTCCAGTGCTACTTAATTGCCGCTCTCACTCAAGGCAATTAAATCTGAACCGATCTTGGTTCGCACTCTCCAGAAAGCCTCCTTCAATAGCCAGCATACATGTGTGCAGAGGATTGGAACACTATCGCATATCATTCAGCAATTGGGGGTTGATGTTGGACATAGCATGATAAG

 

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