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CRIMIC CR70312

Gene CG7744

CG7744

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2R:19452504..19455527	56D8-56D9	FBti0217687

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ATTCCGATGTCCTGACTGCTCAAC	CTACAGTCAGGATGGTCATCAG

Images

Construct Design Sequences

sgRNA sequence	CTAAATACCCGTATCTACATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTAAAGGTTTACAGGTGAGCTGTCATAACAATAAGCATTATATATACAATGTTATGGTATCTATGTTTACTAAGAATCAAGCAAAGTAATTTAGCTTGATGTGTTTTATAAATGAGTTAATCGTGTTTAAGTCAAGCAAATAAGATATACAACAAACATACAAATGTATGTCTGAATATGATCTAAATACCCGTATCTA	CATCGGTTTGCAAATCAAAGACAAGTTAACTAGATTAGATAGTTTCTATGAGTAAGCCGATTTATAATTTATATCTATTAATAGAGAACCTTTAATATAAAAGCTGGGGCACAAGTTATTAAATAATAATATTTATGTTTTCCGTAGAGAACGACTGGAGAAGCAGTTTCGCCTGTGTAGCAGCTGCGAACGACATGTGA

 

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