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CRIMIC CR70315

Gene G9a

CG2995

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:245978..254650	1A1	FBti0226859	97160

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CATACGTACATATGATTGATGTACGC	CCACCTGAACATGTTCATCAGTAAC

Images

Construct Design Sequences

sgRNA sequence	GTCAACAGACCTCTTATGTTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAATATTCTAATGTATGTCATTGTTTTTGTTCAGCTGTTTGTTTAATATGGTTAGGTCATAAAAAGGTTAGATTACAATTGTTAGTTTGTCATAATTCCAGAGGGATTTGAATACAAGAAAGACTTGAAGTAATTGCATCCAATTTTAAGAGGGTTAAGCCATCCATTCATTCATCCTCTTGGTCAACAGACCTCTTAT	GTTTGGTAGAAACAGTTGTCTAGGCAACCGATGAAGGCATTTGAAGCTTTCTTTGAGGACTGGTACTTCTAGTTAGTATGTCTCTATATTATATAATATATAATATTATCCTGAGTACTTCTTTAGTCGGCACATTCAATGAGTCTCTGTACTTTGCAGGTACAAGTTTCGCCCGGTGGAACGCGACGGTCTCGAAGAAC

 

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