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CRIMIC CR70316

Gene MAN1

CG3167

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:24033974..24036707	60B5	FBti0220988

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGATAGGTGAGAACGCAAGCGT	TGCATCTCGACTTGCGAAACAGTC

Images

Construct Design Sequences

sgRNA sequence	ATTAAGTCAAGGGTGCGGCGTGG
sgRNA2 sequence	ACTTTACTTTCACACCAACGAGG

 

left_200bp	right_200bp
GTATGACGTTAAATAAAACTAACGATTCAAGACTGTTTCTCATAGTTTGTTTTTAGTTTCTTAGAAATCTCATACCACCCTTAACGTATTGTACATCAATTGCCACATATACCCTCTAAATAATTGATTAATTGTAAAGAAAAACCTATCGACCGACTCAAGCCGATTCCATTTCCATTCCGTTTCACTTTGTCCCACGC	ACGAGGCAGCCAACACTCACTCATAAAACACACAACTCTACATTAGATACTTAAGTTATTAAATGCGAATTTGTTTTACATGTACAGCTGGAGTTGTCGTATTTAATCGATTTATTATGAACTAGACTTTAAAACTTAAAAGCTGCTAAACACTACCACTACTTTGCTAGGGAGTTGGACGACAGAGCGCTTTCCCCAGT

 

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