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CRIMIC CR70324

Gene Tim10

CG9878

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:21690215..21691139	57F8	FBti0217689	93833

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACAGGCGATTCGATTACTAAGCG	GTACCTCTAACAATCGTAGCGC

Images

Construct Design Sequences

sgRNA sequence	CTTTCTATGACAATCACTTCAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAGATCAGCACCGCCGACCAGGCCAAGCTGCAGCTGATGCAGGAAATGGAGATCGAAATGATGTCCGATTTATATAACCGCATGACGAACGCTTGCCACAAGAAGTGCATTCCGCCGCGCTACTCCGAGTCGGAGTTGGGTGAGTACCATAGCCATATAGAATGCTGTATCTAGTTCGCAGCTCTTTCTATGACAATCAC	TTCAGGTTTCTCTTTGATTCGATTGATGAAATTTAAATAGAAATCTTGAAACTTGTACCACAATTCCACACTTATAATTTTAATGCTCTCCGCACCCTACAGGCAAAGGCGAGATGGTGTGCATCGATCGCTGTGTGGCCAAATATCTGGACATTCACGAGAAGATCGGCAAGAAGCTGACGGCCATGTCCATGCAGGAC

 

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