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CRIMIC CR70326

Gene rb

CG1799

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:4535635..4540626	4C7	FBti0226909	97300

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TTTAGCTTTCTGTTTCCTAATGG
sgRNA2 sequence	GAGTGGAATCCCCTGGAGCAGGG

 

left_200bp	right_200bp
AACTCATAAAAATTCAAAAGCAACATGAATGCCGCGAAATGTTTCGGTTAAAGTAATGGATTTCGTAACTAATACATTACTTTATTTATGTGCGTATCTATACGAAAAACTCTTGGTGCATTCCATCACTGTTAGTTAAAGTGTTCTAGTATTTTTGTGCTATGTGTTAATCGGCGACTATCGACAGTTGCCCACCATTA	GCAGGGCTACTCAACTGAGAATAAGGCAAAGCCGCCTCACTACGTAAACATAGCATTCATATTTAGCTATACACTTGTAAACGGGCGGGGAGAACGTAATCTTTGAAATTAGCTGATAACATTAGCTGAGATTACAGGTGTTTTAAATTCCATTTTCGAGAGATTATATGTAAATGTGCAGTGTAACAGATGCAACTTAA

 

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