CR70327

CRIMIC CR70327

Gene CG10939

CG10939

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:17394593..17405171	54B6-54B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GGGTCAGCATATTGTCCAATGTCG	CGATTTCCACAGTCGTACTTGTCC

Images

Construct Design Sequences

sgRNA sequence	GGCGTGGACATCTTGACTTCTGG
sgRNA2 sequence	TCAGAAGCTCTGAGACGAAAAGG

left_200bp	right_200bp
GCAAATGTTTCTGCGGCACTTGAGTTCCACAGACTGATTGGTGAACCAAATGGAATTGATCTTGCAAAGCATTTTCTGCTCCCAAACAAACATATTTTGGTATTTTTGCAAATATAGTAGTATATAGTATGATTTTTGAAACCAGTAAATAAGAAACTATAAATAGTGTAATACTTAAATATTTTATTTTCTTTCCAGAA	AAAAGGGTAGCCCAACCAACTACTTGTTATAATGTCAGGATGAGGAGCTAGAGCTGGTTTTGTCAGGCATACACCACACCACACAATATACAATATGTTTAGCTATTAGTACGAAGAGTCACTTATTAACTAAGCAAGTTTTTAATTATTACCCCCTAAGAGAAAGAGCGACCAACGATGGTAGAGTAAACGGATATGAT