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CRIMIC CR70334

Gene spri

CG34414

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:10490069..10585422	9D2-9D3	FBti0226901	97301

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCCTCGCCCCATATACC	CGTTTAGATGACTCCATCGCTTTC

Images

Construct Design Sequences

sgRNA sequence	AATGCCAACTGCGTGGTGGGGGG
sgRNA2 sequence	GTGAGCAAGAAGGGCAGAGCGGG

 

left_200bp	right_200bp
CATCAAAAGCAGATGTAGTAGCTGAAAAGTATGCTTTAAAATGATGCAACCTCACCCCCAAACGAATGAAAAACCACCCGATTCATGAGTTTTTATCATCTTCTTTCAACTGCTTATTGAAAAATTGTGCGCAACTCGCACAGAAATCATAAACAACGCCACAGGGTGCACAAATTCCCCCCGAATTTTCCATCCCCCCC	CTGCCCTTCTTGCTCACTAACCAAGCATATTTGATTAATAATTTATTATTATTACATTAAAATATACTTAGTAGCCTGTAAGTAAAGCTAATTAATGCTAATTAGAAAACATCTAATGTGTACTAAACATCAGTATTGAGTTAAGTGTGTTTTTTTTTCCTGACCCACGGGTCAAGCCCCCTTTTTTTTTGCTAATAAAT

 

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