CR70335

CRIMIC CR70335

Gene CG9853

CG9853

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:4398381..4402027	A2A5	FBti0220991	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGACATTGGACATTGCAAGCAG	CGACTATGGGATGTATCGCGATGT

Images

Construct Design Sequences

sgRNA sequence	CAGACATTTGGTTATTTGCGGGG
sgRNA2 sequence	TTAATGAAGCATACCGAAGAGGG

left_200bp	right_200bp
AATTACCAATTTATTGGTTAGAAGTGTAAACCCTCAATTTTTGTTTTGACATTGGGCGCCTGTGCAAAGAGTGTTATAGTTGGCAATCGACACCGATAGTTTGTCATCGGGAAGCTGGTATATGTAAATACAAAATAAATTATAAAAATTGGCTTCCACATAATTGAGAAGGTCGCTGAAAGCGTACGAATAAACCCCGC	AGAGGGATCCTTTGTTGCTGGTCTTTTTGGTTGCTCAAAGTCAATGAAGTTCTTTGCCACAAAATCATCGGGCTATTTTGTTATAACTTTAAATTGCTGTATGCTAAATAATTTTAAATAGGCGTATGGTCTTGATTATGCAAATTATAAAAAAATAGTTATTCAAACAAAAATCACTATGACAAGTTCTCAAAAATCTT