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CRIMIC CR70336

Gene dop

CG6498

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:15566013..15576313	71E1	FBti0220956

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGTAAGAGATTATGCCATCCAC	TACTGGTAACGGAGCGGTCAGTT

Images

Construct Design Sequences

sgRNA sequence	TTTAATCTGAACTTGATAACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATATATATGCATACATATGTATATTTCCGCAATAACGATAATATTAGCTTTGTTATTTATATTTGTAGCATATTTACAAGCTATTTTTATCCTTTTAGTAAGATGTGAGTTCTGTCTTAATTAGCACTCAAAAATTGACACCTATATTTTTGTATTAATCATGTTTGTCTGGCTGCCCTTGTAAGCGTATAAAACCAGTT	ATCAAGTTCAGATTAAAACGTATTTATTGAATTTCTTTTAAAAATTGTCATGTTAAGTCCACTCAACTTCTCCAACAGTTAGAATTATGGTTTCTTGTCCAACTATTACAATTAAAATCAATTGTCAACTATCAATACAAAAAACGTATTAATTGTTCATTGCAGAAACGTCCAACTTGCTGCGTATGCGTCACTCCTCG

 

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