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CRIMIC CR70339

Gene DptB

CG10794

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:18867391..18867895	55F8	FBti0220992

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCAGGTTACCAGAAAGAAAGTTGGTG	CAAGCTCTTGACACACATCCAGAC

Images

Construct Design Sequences

sgRNA sequence	TAGGCTTTGTCAACTGAATGCGG
sgRNA2 sequence	TGAAGTGCCCTAAAACCTGAAGG

 

left_200bp	right_200bp
TTTCTAGATGGGATTCACTTTGGCATCTGCCTCTGTTTGAGGAGCCTACACGTACCTCTTATCAACGAAATGTGGGGATCCACTGGTGAACCACTGCACCGTCGTCTTGACAGGGATTCCCAATTGGGGAATCTCATCTGCGAGTACTGATAAGACACAGATCCGAGCTATATAAGAGCTGAGTTCGACTCTAGCCGCAT	GGTTTTAGGGCACTTCAGAAGCTACATAATTTTTATTAAATAAAATAAATTTATTACAATTACAAGTCACTTTTTTTTTTACAAGTATTCAGTTCCAGATATTCTCCCAGGGTGATGATCCCTTATCAGTTTGATTGTAGGCAAATATTGTGGGATCTGGCTGAGGCATGAAAAACTATAGGGAGAAATACAAAAATGTA

 

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