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CRIMIC CR70340

Gene Dif

CG6794

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:17413248..17433161	36C7-36C8	FBti0220959

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGCCTAATTAGCCCATCCAG	CCCTCCAAGATTAAATAGAGTGCCC

Images

Construct Design Sequences

sgRNA sequence	CATCAGATACGCATGTATGAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATAAAACGCCAGCAGCAGCAACAACAAATATCGAAAAAGTGGCATAATAAGACTAGCAAATAGCTGCGCAAGTTTTGACGTCAGACCTCGTCATCGCATAGTCGACATCTGGCAAAAACAAAGTTGAATTGAAATGGAAGAAGGAAGCCTTCGAGCCATCACCCTTCCGCAATGAACAATGGCAACCTAATGCCCCTCA	TACATGCGTATCTGATGGATACAAATTATACGCAATTTCACTTTTCCATTTCGATTCCGCAGGGTAAATTATGTTTTCTCGGATAGACGAATAAAAACTTGTAATAGTTCAGTTTTCGAAGCACTTTAAGAATTTGTAGTATTCTGAGCGGTATTTACCATTCATAATACTTTCTTGACAAAAACCAGGCATAAAACTTT

 

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