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CRIMIC CR70342

Gene Tim9a

CG1660

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:13385210..13385893	11F1	FBti0226860	97161

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATAAGTCGAGAACACGGAATGG	CAGTTCAGCGAGCACTTCTCC

Images

Construct Design Sequences

sgRNA sequence	CATCTCATGCCAAGGACCGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTGGCAACCCTGCACACCCAGCTGTCCCTATTATATAGGCACATTATTATTGGCCCTTTTTCGTTTCGGCAATTTTGAATAAAATATCGCAGAACAAAATGGCTAAGACACCGGAAAACATAGCCATCGATCAGTTGGACAAGGATCAAATTAAGACGGTAAGCAGAGTTGACACGAAGATACCATCTCATGCCAAGGAC	CGGCGGTGCCAAGTGATGCAATGTGGTTTTCATTTGATATGTTTTCGGTTCTAACTTTCAGTTTTCCGACTTCCTAATGTCCTACAACAAACTGTCCGAGACGTGCTTCACAGATTGCATACGCGACTTTACAACGCGGGATGTTAAGGATTCCGAGGTGAGTGCCCGTGGGGAAGCTCCATGTGGAAATCAGCGATTTC

 

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