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CRIMIC CR70345

Gene Rel

CG11992

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:9044183..9047977	85C3	FBti0220960

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATGATTTGCACGTGTCCAAGGAG	TTAAAGGCCTCAAAGCAGAGCC

Images

Construct Design Sequences

sgRNA sequence	TCGAGTTGCTTGCTTCGATGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATACACACCGCCAAGAAGTACATATTCGAGGAACTGTGCAAGAAGAAGCAGGATCGCCTGGTCTTTCAGATGAACCGCCGCGAGTTGTCCCACAAACAGCTACAGGAACTGCATCAGGAGACAGAGCGTGAGGCCAAGGACATGAACTTGAACCAGGTGGGTAAACCAGCGCGTAAACAATATCGAGTTGCTTGCTTCG	ATGAGGTGAATATGCACGTTTGCATTTATTATTCACACGTTTAATTGCTCACTTTAGAATGCTTTAGTCAATTAATTATAGGTTGCATTTGCTACCTTTTAATCTTTTTATTTAAGCAGTCTGTAAAGTATCTACTCGTTTAGCTCGAATTATATAAATTTCTCTGCTAAAATAACTGAGCAATATAACATCGAAGCAAT

 

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