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CRIMIC CR70346

Gene HDAC1

CG7471

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:4626734..4629467	64B12	FBti0217692	93836

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGCAAGTTCATCCGATCGACGAA	TCTGCCTGAGAGCAGCACTTTT

Images

Construct Design Sequences

sgRNA sequence	TGCTATCATCTGATGCTAAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATCGACTTTAAGGGGATTTAAGACCCGCCAACAGCACCGCAAAATGCAGTCTCACAGCAAAAAGCGCGTTTGTTACTACTACGACAGTAAGTTTTGAGCATTTAAATGGAAGTAAAAGGCCTACTAGATGGGTAATTTGTGTTTACAAAGTGCCAGCTAGGGTTACCATGTCGACTTTGTGTTTTTTCCGCATCCACTT	AGCATCAGATGATAGCATTTTTGCAAATTTCAAGTTTTACGGCGACTGTACGAGCGCGTTCCGGTCCACAGCGCACTTTTTCCATACGACCGCAGTGCGTTTGTATGCGTTTGTGTGTGTATGTACCTGATGTCTATCTTCCTCTTCTGTGTGTTTTTGTTGCTTTTTCGTTCGGGGCGAAAGTGCTACTACTGCGGTCG

 

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