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CRIMIC CR70347

Gene Spred

CG10155

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2R:14759646..14769423	51C2	FBti0220961

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGAACGTCGCCATAAAGTAG	CCAATTTCCCGACTTTTTAACACC

Images

Construct Design Sequences

sgRNA sequence	GGCTGTGTGCAAATATAGGTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TACACACGAAGTAAGCCATTTTATATCCTACTATTGTTGTAACAAAAAAGTCACCCTGTTTGCTGCGTTTCTTGCTGCAGCGGAAACAACATTCGATTTCAATTTTGCATTGTAAATTGATTGGCATGTGAATAATGTAACGGTAAGCGGGACATCGTTACCACGCGACATACATATGTATGTGGCTGTGTGCAAATATA	GGTAGGAGTGTGCGTGTGTGTTAGACATTGGGGGCTATCAAAACTATTCGCCTATTTAAAGAGTGGCTCTGGGTGTTGAAACTTTTTATTTCTTGTTGTATTGTGGCGTCGCCTCTCCCACTTTTCGTGTGGGTGTGCACGAACACAGGCATAGACGTACACGCACACGCAGAGCGTTCATTCATCTTTTCCTGCTGCAG

 

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