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CRIMIC CR70348

Gene Rop

CG15811

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:4136359..4138996	64A7	FBti0226910	97302

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCACACTCGCACTGAGAC	CTGTGTGAGTTGCGTGTCCC

Images

Construct Design Sequences

sgRNA sequence	TTTATTGGTCACGGGCGCAAAGG
sgRNA2 sequence	AGTTTGTTATTCCTTACCGGAGG

 

left_200bp	right_200bp
ATATCGATATCAACTATCGCTAGCCAGCAATCGACTTTCGCCCATCACTAAGGCTAAGTCATCAAACGAAAAAAATACAGAAAAATTGGCCAAGGAATAGATAAAATTCTTTAAGTCAAGTTAGCCCACGCGAAAAAGCGAACGGAATAAGCTAAAAAGACATATTTACACGCAGTGTGTTCATAATCGAGTTGCCTTTG	CGGAGGTTCAAAATGATTCCTTAAGAAATTATTATACTAATTACATATTGCAATATATATTAAAAATACAATACATAAATTATATACGCATTACATAATATATATATTACAAATATATAGTATTTTATTCTTAGCCGAATATAAATAAATATTAACTTCAATAATACGCGTAGTATTATTTCAATTAAGCCCCTATACTG

 

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