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CRIMIC CR70349

Gene wrd

CG7913

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:18167757..18180049	90F4-90F5	FBti0226902	97303

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTGAACGTGAGGAGCTGTTC	GATCGGTGTTCAGGAACACGG

Images

Construct Design Sequences

sgRNA sequence	GAGAACAGATAAGTATGTTGTGG
sgRNA2 sequence	AATTTGAATTGTCTAACTTAAGG

 

left_200bp	right_200bp
AATGCTGCACACTGTTCGACTTCTCCGAGCCGCTCAGCGACCTCAAGTTCAAGGAGGTGAAGCGGGCGGCTCTGCACGAAATGGTCGATTTCCTCACCAACCAAAATGGCGTAATAACCGAAGTTATTTACCCGGAGGCGATCAATATGGTAAGCTACTAATTTACATATTTAAAATAATATCACATGCTTTTACCACAA	TTAAGGCGTAGGCGTTTACCACCTTGATTGAGTACATATATATCCCCCGATTTCTGCCACATGCATACATAGATAGCTATATGACATCCAGACATTCATGCCACATGCCACTATTATACACACATACTATATAAAACATGTTTACTACTCCACGACTCATATTTGCAAATATTGTACGCTATTTAGCCTAGCAGTTAGAG

 

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