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CRIMIC CR70350

Gene CTCF

CG8591

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:7353578..7356713	6F56	FBti0220994

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGATCAGTACCCATGAGCG	CAGCTAAACGCAAACGCACC

Images

Construct Design Sequences

sgRNA sequence	TGCCCATTTCTAATAAAAGATGG
sgRNA2 sequence	GAAATACGTTATTGTATGCTTGG

 

left_200bp	right_200bp
CGCTTACGCAGCCCAAATCCTTGGCTTATCCAGTATCCAAAAGAATACAGATTATATTTTTTATCTAATAAATTACCGTGGATTCGTTATTATATATTTTCTGTTATCAAGAATTAAAGATAGTTTATATAGAGATGACAAGATACGAAACCAATCGAACTATCGATAGCTGGCGGTACTGATGCTACATGTCGCCATCT	ATACAATAACGTATTTCTACGTTACATTGTTTTCTATTTAAATACACATTTAAAACAGTTAGGAAACCTATTTTTACTAGGTTAAGAAATGGATATTAAAAATGTATTCTTTCTAAATGAATGTATAATTCTGGATAAGCAGTTAACATGCCGATATGGCGACTATTTGTAAATGATCCCCGACACTTAAACTATAGTTG

 

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