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CRIMIC CR70351

Gene FoxP

CG43067

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:9726020..9730872	85E5	FBti0220995

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACTGAATTCATTTAGCACTGGGC	GAAGGAAATTAGATAAGTCAGCAGAAAAG

Images

Construct Design Sequences

sgRNA sequence	TATGCCCATCTCTTGGGTAAAGG
sgRNA2 sequence	TATTAAAATCGTGTTATAAGTGG

 

left_200bp	right_200bp
GTATCATCTCAATGATAGTGCGGGAAAATTAAGCTGAAATTAAATAGGGCGTAATAAATAGAAATCCTTAAACTATCACTCTGATTCGGAGTTTTACAGGAAAGGCGGCATATGAGTAAAGATAGTTTTATTGAAATGACTCCTAATGACAGACGAAAAAGAGATTATTTCTGTGTATTTGCATATGCCCATCTCTTGGG	ATAACACGATTTTAATATACGAGTACAACATATATGAATATTTTCACTTTTCAGCAAGGGAGAAAACATTTAAAAGCTATAGCTTGATAAAATTTTAACAAAACTGTTTTAAAAGTCACAATATGATTTTTTTTTAAGGAAACTATTTTCAGGTATACCTTCTGTTAAAAATCTGGACCCTTGAGAGCTTTAGATTTGGA

 

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