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CRIMIC CR70352

Gene AttA

CG10146

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:14747362..14748215	51C1	FBti0220996

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTGAAACTGGACAATGCAGCAAC	CTATTAACAGGCTGGAACTTTCAG

Images

Construct Design Sequences

sgRNA sequence	ATGTTGCTGAACTGGATTGCTGG
sgRNA2 sequence	CTGGGTCTATCTCATCATTTCGG

 

left_200bp	right_200bp
GGTCAAGTCGCACAGCCACACAAGCAAACAGCAGAAGTAAACCACCATCACATCTGAGCGGGGAATTTCGCTTTGATAAGGCATCCAGGCCGAGATCGGCAATCAGATGAATCATGTCAATCATCAGAAAAGTTCTTCCCCGCATCTTGAGGTATAAAACCGATGCATTGGACACCTTGAAACATCAGTCAGCTCCAGCA	TTTCGGCTAAGCCTGCGTATTTGATATTATTAATTCTAATTTATTGACAATTATGTTAAAATCAAAATATTAAAACCTGAAAAACCTAATTATATATTTTTATTTAAAAAATAATCATGTCTTTTTGCATAATGTATGATAAGCTTAATTTAACTAACTTGATGAATCAAAATCATCATTATCATTAGAATCAATTAGAC

 

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