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CRIMIC CR70353

Gene AttC

CG4740

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:13393705..13394667	50A3	FBti0220997

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACTTGCATCGCGTACTAATCGA	AGTCAGGATGTGCGATTGCTCGAT

Images

Construct Design Sequences

sgRNA sequence	GACTGTTGACTGACGATGCTGGG
sgRNA2 sequence	CTGGGACTCTCGCACTACTTTGG

 

left_200bp	right_200bp
AGTTTCAAGGGGATGCACAATCTCTTATCAGACGGTGTGAACCACCTGGTCATTCGGGGATCCCAGTGCGAAACATTGCTCAGCTTACAGATAAGGCATCACAAATCTTCCATCTATAAATTGGTATTCATTGTCGGTTTCGATGGGAAGCCCCACTCAGAGATAGATTGCTATATAATTCACGGTGACCAGATCCCAGC	CTTTGGATAAAACTATATTCTTAAAAATATTTTACAATAACTAAGTGTTATTCAAAATATTTATTGACATAAATTCAAACGAATTTTGTTGCTTATTTCAAATGTGTTAAAAATATATGACTTAACTCAAACAAAGGGAATTTTTAATTGAAAAAAACAACTAATGAATTGAAACAAAAATCTAAAACGTTGATTAAAGC

 

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