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CRIMIC CR70356

Gene CG4960

CG4960

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:25786329..25787298	96F3	FBti0220998	95051

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATGGAAGCCATGCAAAGCTGTG	GCATTCTTTCGAGTTGCATAACTTGCC

Images

Construct Design Sequences

sgRNA sequence	ACTGGCATTTAGAAAGCCGGCGG
sgRNA2 sequence	TGCGAAATGTATCCGATACGAGG

 

left_200bp	right_200bp
AACTAGGCAACCGTTTATTATACTTAATGGAGGAAGAGGAAAAACAGAGGTAATTTCTATAAGAAAAAAAAGAACGGAGCTGATTCAAAAATATAAATTTTTAAATTTTCTATACAAGATACCATTTTGCGGCTTTTACCTTAATTTAAGGTCGTTTTTGTAATGCAAACAAAAAAAAAATCGACTGGCATTTAGAAAGC	ATCGGATACATTTCGCAATAAATTTTAATGATGTATTCATTTTGTTGTTGGAAGGAGCACCAGTGGTCGCTGAATCCTAAAAAATCTATCCTGGTTAAAGGTAAAAATGAAATCTCACCACTTGCGCGGCTGCTTTTGTGGACTCGAATTTAAACTCGAAGAGGTATTCCGGCTCAGAACGGCACTTCCATTCTTTAAGT

 

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