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CRIMIC CR70357

Gene CG5250

CG5250

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:19228217..19229276	91F12	FBti0220999	95052

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGCAGGAATCACATTATCGC	TGTCGAATATCCTCTTCGACCACG

Images

Construct Design Sequences

sgRNA sequence	ATTTCCATAAGATATAATTAAGG
sgRNA2 sequence	GTGAGATCAGCTAACTCAGCTGG

 

left_200bp	right_200bp
GATTGCGATAAAATACTTAGTGCTGCTACGAAACAAAGGGCTACAGGAGTTCACTTAACGCCTATCGTTATCGACAAAAAGGCGCGTTATTCAAAAATATACATTAGTGCTTCTGATATCTGTGCGGAATACATTCCTATATTTCTAATCTACCCCAAGCTAGCTTTCTGAAATCCCTCATATATTTCCATAAGATATAA	AGCTGGATTTATTAATGTATTAATAAATACTTAACAAAATAATAAAACTATTTGAGAATTATAGTTTACTCCGCTTTAGATTTCATAGTGAACCGAAGCTACAAGCTTCGAATATTTCCCACTTACGCCCACGTAACCAGTAGCCAAATCAGAACCGGAACGATCGCCAGGATGCCGGAGGACGTAATGGTGCCCGCTGG

 

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