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CRIMIC CR70358

Gene CG8728

CG8728

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2R:7936866..7939114	43F3-43F4	FBti0220962	95053

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATCGAACTGCAGTGAGCTGGCAA	GCAGCGTCGTTACCTTCGTAAT

Images

Construct Design Sequences

sgRNA sequence	AAAGTGGGCTGTTATGGAGCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAATTCATTCGTTTAAAAGCGTAAAAAAATGTGTTTCTGAATCAAATTCAAATATAATTCACATAATTCTGTAAATACCGTTATAATACCCAAATCACCAACACATGATGAACGGGCGCAGCATCGGCATGCTGAAAAAGCTCAGGCACACCTGGCGCACGTAAGAATCTCGCGGAGTGGTGAAAAGTGGGCTGTTATGG	AGCCGGCGAATCTAATAAGTGCCCTATCTCCAAACACAATGCAGGTTTCCACGCCTTATGGCCACCAAGGTGACCAGTCTGGGTGGCGACGAGGTGGGCAGTGGCACAGGAATCGGACAGATCACCGGCGGCGTGCGTACAAGTGATGGGCCACAGAAAGTGAACACGCCTTCCAAGGAGATTGTCACCCACCTGCCACC

 

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