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CRIMIC CR70359

Gene CG11975

CG11975

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:8993253..8995212	85C1	FBti0226912	97305

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGTAGTGATGCTGGCAG	CGGCAATGGTAATGAAAATGAGTAATGTC

Images

Construct Design Sequences

sgRNA sequence	GATGCTGGAAGCCCGGGCTCTGG
sgRNA2 sequence	TATACCTTGAGACATGATTGTGG

 

left_200bp	right_200bp
TAGCAAGAAGTTCTTAAACCCAAAAATATCCCGATTTAGCGGATCGTCTTTCCTCATTGAAAGTTATCGATTCACCGCCCGGCCACTTTTCGACGATACTCAACACTGTGTCATTGTTTTTATACGAAAAAACACAATCATCCGCCGTCCGAGCAATTGTTTTCCTGCCTGCCCAGCAACTGTATTGAAAATCACCAGAG	TTGTGGATATTTTATATTTTATTTGAAACATAAACCGATAATCCAACATGCTCATTTAATTTTACTTTAGCCTTCATTTTATGCATTGCTACGCCAATTGTAACTTTAAAAGCTGAAAAAGGTTTTTGTTTTATTTCCACACTGAGAAATTAAGATTTACTTATTAATTTTAAATGAACTTGAGCTTATCGTTTTTGTTG

 

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