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CRIMIC CR70361

Gene Cyt-b5

CG2140

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:7533506..7535936	43D3-43C5	FBti0226861	97162

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CAGCATGACTCTAAGCTATAGTACAAAGTTTC	CCACGCTTGTCCTTTCGC

Images

Construct Design Sequences

sgRNA sequence	TGTCTGGTCCATGAAATTCAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTTAGGATCAAAGTTTCTGTAACTTTATTGGCTTATAAGTGCCAAATTAAATCTGTAACTGAAATTAGTTGTGATATTTTTACTTTCAACGCAACGGAAGTATAAAATATTACCATAAAGCTTCCGCATAAACGAATGTTTCGAAGCATTCCCGACTTTATACCTTGATACCATACCCTCTGCTGTCTGGTCCATGAAAT	TCAGGGTCAGACGTCCATTAGCATTTATCCAAGATCAGCCAACAGTTATTTATGCGAATATATTAATCTGTCTCTTTTCCTTCACGACAGCATCCCGGTGGCGAAGAGGTGCTCATCGAGCAGGCCGGCAAAGATGCCACGGAGAACTTTGAGGACGTTGGCCACAGCAACGATGCCCGCGACATGATGAAGAAGTACAA

 

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