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CRIMIC CR70363

Gene ReepB

CG8331

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:14170462..14172998	50E1	FBti0220964	95055

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTGAATTTTGTGGTTTTGTTTTGAGCAAG	GCACCTACATGACCGCCTG

Images

Construct Design Sequences

sgRNA sequence	TATTATATTTCATCATATGTAGG
sgRNA2 sequence	TAACCAATACGAGGTGAGGTTGG

 

left_200bp	right_200bp
CCCTTGGACCTGCTTATAGCCATAAGGGAAAATGCGCCACTAGGGCAATATCAATTGACCAACTAGATGGCGAACATGGCAGTCGAGGAATTAGAGAGCAAAATTGTCCAACTGTTCGTAGAGAACCCCCTCCTGCGCTACGGTAGAGCTCCAGCAGTGACTTAATGCTATTTACTAATAAGGTATTATATTTCATCATA	TCACCTCGTATTGGTTACGTTTTGCAATAAATTTTAATTCTGTATTCATTTTGTTGTTGATAAGAGCACCAGTGTTCGCTGAACCAGAAGAAATCTCCGTTTGTTAAACGTAAAAACGAAAAGGAGGCTTTTATTGAGGCACAACTATATTTATGCGGTTGATTCCGCCTTTTCTGGCGACGCCTCCGCTTCGGCAGCTG

 

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