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CRIMIC CR70364

Gene Reepl1

CG11697

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:11572707..11573640	10C7	FBti0226913	97306

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGATCCAGTGATGCCACATC	CGGCATATCATCTCATAAAAGGAAGGAC

Images

Construct Design Sequences

sgRNA sequence	TATATATATAGCTGTGTATTCGG
sgRNA2 sequence	ATTGATATCCTCAATATGTCAGG

 

left_200bp	right_200bp
ACGATATAAAAAATTGCACTTTTAATATTAATAAAAAGATATATACTCATTGAAAAAAAGCTGAACTAAAAAAGGGGAAAAGGGTTATAGGGCATAAGTCATTTTATGAATGCTAAGCAGGCCTTGCGTTTAGAATCACATTTCAATTTATTGTATTTGTTCAAGTAGTCAAACCAAATGAACAAAATCGAGAACCGAAT	GTCAGGCGGAAGTTGTCAAAGTTCAGAGCTTTCAAAAAGCATACATCTCAGAAATAAACTAACCTTACCGCAATAAAGTTTATACAAAAAAAGCATTTAAATTGATTAATTTTAGTCACAAAATATTGAACCAATGTGTTGGTTATCTTCTTAGCTATTTTTTCTAGCCTATTTTCTTGTGTTTGCACAATCAGTCGCGG

 

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