CR70365

CRIMIC CR70365

Gene tbrd-2

CG7229

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:19259909..19262294	56C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	no band	CCCTGACTAGAACTTTCGAAACTTTC	CCCTGACTAGAACTTTCGAAACTTTC

Images

Construct Design Sequences

sgRNA sequence	TGAAAAATATATCTCTAGCATGG
sgRNA2 sequence	ATTCATAAGACTTCTTTATGAGG

left_200bp	right_200bp
AGCTGAGCGGTGATATGTGTTTTTCCATCACAAAAATTTGTTCCTGAAAGCGGACGGTCGAACGGCAAAAGTGTTCCCAAAACTCAATCGTAATCGTAGTCAAATTCGAGTTTATATTTGTGTAAAATTGAAAGAGACACGTTTTCAAAAATCGAAACAAACGAACAACCAGTAACCAGTAACTGAAAAATATATCTCTA	AAAGAAGTCTTATGAATTCTTGCATTTTATGTATATGTAAAAAAAAGGGGGGTTTCACTACAAATATCCCAAATATAAGAAAATAAAAACTGTAAACACCTATATTACACATTCATGTGTACATATATGGTTGGACAAGAAACTAAAAAAAATTAATATGTTTGTAATTCATTATTTCCTAAGAGCTTGGGAAATTGCGA