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CRIMIC CR70366

Gene ZnT86D

CG6672

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:11212308..11214539	86D8	FBti0221001	95056

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTGAATCGCAGATCGACGAG	GCTCCTCGATATGGACTATTGC

Images

Construct Design Sequences

sgRNA sequence	CGGTATCATCTTGCCGGTTATGG
sgRNA2 sequence	CACCGGTGGGTCGATCCCATTGG

 

left_200bp	right_200bp
ATCAAAGAAAAGAGACCTGCCGCCGCCGCCGTTGCCGATAAAGATTCTCGCGAACCCGGCCAGAAGCGGAGTGGGAATCACGGAATCAGCTGGAGCGCGGCTATAGCCAACCGATAATACCACGTATGCAAACAAAGTGTGCTTTCTTAGTTAGGTGCTTAGATCTCATGTTGGTCTCGTGCTCTTTTGCAGATCCATAA	GGATCGACCCACCGGTGTCCTTGTACAATGATCTGTATAGTTATCAGCGCGTGTTGAATGTTTGTGGGCAGTGCGACTCAAACATTATGAAATTTTAGTATACTACGGCGCCACAATACCCCGCCCATCACTTCCGAATGTCCCCTCCAAGAGCTAAGTAAAAGAAAGTGTTTGATTTTCAGTTGTACTTTCCGAATTTT

 

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