CR70368

CRIMIC CR70368

Gene CG6664

CG6664

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3:17012886..17016180	73E4	FBti0221002	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACTTCCACCTTACAACGACAAG	CCACATGCGAAGGAATTGTGGGAA

Images

Construct Design Sequences

sgRNA sequence	CGGCGAAGGAGTGTTCAAGAGGG
sgRNA2 sequence	GATGCAGTAGCTCTCGCACAGGG

left_200bp	right_200bp
CTGTGGATTTTTCGCAATTTGACTTTAATTTTATTCGATCGGATCGGGCAAAATGTACCGCACTCAGATCTGCAGGCCCTAAGCAGACGCCTAAGGTTCAACTCTACTCCGTTCGCAGCTGCATCCTTTCACCTGCGCAGGTGAAACGCAAGAACGGCAACCAGAGCCCTGGTGAAACAGAGTCGGCGAAGGAGTGTTCA	ACAGGGATACAGAAAAATCATGACTTATTCACACACTTTCACACGAAAACACACTATATTCATAAACACGCACGCATGCAAGCAACCAGTTTAACGATCCTAAAAACATAAGAAAACACAGAAACAAAAGATGAGAAAGTAACTATCGTAAGCTTCTCCAGGGCGACTCAAAGTCGCCACATTTATATATGGTTAATATG