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CRIMIC CR70370

Gene CG31454

CG31454

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:8521658..8523520	85A2	FBti0221004

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCATGGGTGCGATAAGCATAAGT	CCTACTAGGAAACCACGTTATGGC

Images

Construct Design Sequences

sgRNA sequence	TCATTTCCGTGTGTTCAAATTGG
sgRNA2 sequence	TGGAAATAGAGGGTTCGATGAGG

 

left_200bp	right_200bp
GAAGGTTACGCCGAAAATGAACAGAACGCGGTTCAACTGATAGTGGATTACCTTTAGATTAATAGTTGGCATATTTCAAATAATACTAGTGCATATGCATGCAAATTATCTGAGCCAATTCAAGGGTTGTGATTAACCTTATCAGTCGTCTGCATTCGATAAGCGAAGCCAGAAGTTCCCCAGTCATTTCCGTGTGTTCA	CGAACCCTCTATTTCCACTGATTGTTTAGAAGGTCAATATATATTATATAAAGATTTAATAATTTGTATTATAATTTTCAAGTTTTCTAATGCTATGTAAATATATGAACTTTCCTATCCATGTAATTAAATTCGTATACGTAATTTATTATAGACCTTTAAATACCTTTTTTGGGGTTTGCTGCAACTGGGAATGACCC

 

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