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CRIMIC CR70372

Gene CG4554

CG4554

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:22483303..22492033	58E3	FBti0221005

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGGAAACCAGCAAACGATACGTG	ATCGATAGGGTGGCTGTGCAATGA

Images

Construct Design Sequences

sgRNA sequence	CACTGTTATCGATAGACAGTCGG
sgRNA2 sequence	CTAAAGGCGGCTGTTGTGCGTGG

 

left_200bp	right_200bp
GTGGCGCATATTACAGCGAGCTGTGGAAAATTGCATCTAAAATGTTCAATTAATATTCAATTGATTTTTATTTAATGAAGATTCTATAAATTTAAATTGTTTCGAAGACCATTTAAAAAAGTGCGCCATTTCGGAACGATATGCTGTACTATCGATATTTTCAAGCGAACATATGTTTTCAGTAAGTGCAATCGCCGACT	GCGTGGCAAAGCGCCGGACATAAAACAAAAGCTGAAAAAACGCAAACGAAAAGCTGAGATGGATGGATTTTAAATAGTTAGTCGGTCTGCTTTAGTATAAGTTTTTTTATAAGTAGAATGAAAATATAACAAACATTGTTACATAAGCAAAATGAAACAAATATTTGACGATTTTGCAATATCTAAATAGATTATATTAT

 

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