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CRIMIC CR70373

Gene Emc1

CG2943

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:7903030..7907363	84E4-84E5	FBti0221006	95061

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGTGAAGACCGAATGGGTTTG	CTTTACCGTTGTCCACCTTTACAAATGC

Images

Construct Design Sequences

sgRNA sequence	TAGTCGAAGGTCAGCAAGCAAGG
sgRNA2 sequence	TACTGCGACCAAATACCACAAGG

 

left_200bp	right_200bp
GCCGCACTGCTTTGAATATCGGATGTGAGTCCGCTGAACTATCGATAAGGCAATCGTCAGCCAACCCTGCTCCAATTTCGTCTTCTCATCCCTTTTGCCATGACGGAAAACACGAATTGATGTGGATTTCTACTAATTTATAACATAAAAATACGTCAAAAGTTGAACAATCAGAGATCGAGCTAGTCGAAGGTCAGCAA	ACAAGGGAGGATGGAATGATGATGAGAGAATGTAAATCGGCGAGAGACTATTGATATATATAACAGAATTCTGATAGCTGCATTACATCCCACAATGCGAATAAAACCTTTTATCAACGCTAAGCCTAGGCAATTATTTATAACTAATTATATAGCGTTAAACATCAAATAACTATAATTAGCTGTTGAAAGGTTTTCAT

 

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