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CRIMIC CR70375

Gene AttB

CG18372

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:4749223..14750165	51C1	FBti0226914	97307

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAATGGGTCATCAAATAAGAAAGG	GTCAATGGGTCATCAAATAAGAAAGG

Images

Construct Design Sequences

sgRNA sequence	GCTTGTCTTCTGCATGTTGCTGG
sgRNA2 sequence	TAGTTTCTACATCTATACCAGGG

 

left_200bp	right_200bp
ACAAACACACAATAATGCCACACGTAAATCACCCATCAAAACGCTGGGGGGAAGTCCTAACTGATAAGGCAAGCCAGCTGAAAACGACACCGAGATGAATCTAGTGATTTACCAACAAAGTTCTTCCCCTAAGATTACGTATAAAAGCGGATCATCAGCCCACCTTGGACATCAGTTAGCTCCAGCAATCCAGTCCAGCA	TATAGATGTAGAAACTAATTTATTTAAAACAAAATAAAAAATAATCAAAAAAGCTATGAAGCTATCTGCTAAATTATTACCCTTCTTAAAATCAAAAAAAAATATATTTCACTGAGCAAATAGAATATGAACATCCATTTCAAAACCTGGATTAAATATACGCTTTTCGTATCAGTTTACGGTTTTCTTTTATCATTTTA

 

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