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CRIMIC CR70377

Gene CG4770

CG4770

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:19918507..19920584	92B6-92B7	FBti0226916	97309

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGAGTTTTCAAGTTCATGGCTTTTG	CACCGACTCCCGCTAGAAC

Images

Construct Design Sequences

sgRNA sequence	GCTTCAAAGTGGCCACACCATGG
sgRNA2 sequence	TTGAGTGTTTAGCGATAAGCAGG

 

left_200bp	right_200bp
ATCGAAATATGATGTAATATGTTTGTGCTCAAATGTTACGTCTGAGTTGCTATTTTCGTTGGCCAATTGCATGTTCCACTTGCATTTTCACGAAAAACTTGTTTCGGGAGGACCAAGGCATTTCTTTTGCCAAGCCAGTTAGGCGTTGAAAGTGAGTAGCTCACCGGATCAGTAGCTATCGTGGCTTCAAAGTGGCCACA	AGCAGGTAAATTAATAATGAGTGTAAAAGGAGTTGTTTTAAATATTTATTTATAATATAAGTTCCAGTCTCACTAGTTCTCTTTTATGCGCTTAGATTTTGAGTATAACTTTCTTTTTAATGGTCAAATGCCATCCATAAGAGCGGAATCACTATTTAAGATCTTGCCCATCTCATTGTATAAATCGTTCCCTGACTGGT

 

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