CR70378

CRIMIC CR70378

Gene cindr

CG31012

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:30808617..30827005	100A6	FBti0220965	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTATTGCATCAGAATCTAAGTGCC	CCCATCAGCCACTGATAGTTGAAC

Images

Construct Design Sequences

sgRNA sequence	ATTGGCAGGCACTTCAGTGTTGG
sgRNA2 sequence	TATTCTGTACAGGCGGGTCTGGG

left_200bp	right_200bp
CCCCAATCTCTTTGATGCTTCCGTCCGACTGTCCGACCATTTTTTTTTTTTTTTGTTATTGCGGTGCGTGGCTGCAGCACTTTTGCCACTTCATCACCGGATCTCGATCTCAGACGGGCTCGGTGACTTTAACTGGAGTGAAATTACTACTATGCGCTGTTGACGAAACTGCTTTGGATAACGATTGGCAGGCACTTCAG	CCCGCCTGTACAGAATATTCCTTTTTCTTTTGTGATAAGCCTCCGCATAACCAGCTTTGTAGTCTAATCAAATCAATCTGCGTAGCTAGCCACCCGCAATTCCCGATTGTTAGCAAATATGTTGTGGTTTACTTGGAAATATCGCATTTTGTATGGCTTAACATGTAAATATTATTGTAAATTAATATGGGATATTAAAT