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CRIMIC CR70380

Gene CG11737_CG31259

CG11737_CG

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_SA_kG4	3R:0..0	85A2	FBti0226964	97310

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	CAGCTTGTACGTTCGTAGCTCA	TCTAGAGTTGTCACAGCGGTGA

Images

Construct Design Sequences

sgRNA sequence	TTGAGAGAGATAATCGAGCCTGG
sgRNA2 sequence	ACAGTAGGTATGCGAAGTTATGG

 

left_200bp	right_200bp
GTTTTTATTAAATTTTGGTAAATGGGTAACAACTATAGTTTCGTTACTACAATCTTACTAAGGTATGGTAGCTTCTTATCATTCACAGTGCGTGCTTATCAAAATCGAATGACAATGGTATCATATCTACTATAATTAACGTATTAAAGTGGCAAAGTTTTAATTTATAAATGTTTACCGAGTTTGAGAGAGATAATCGA	CTTCGCATACCTACTGTACAGTAAAAAAAAAAAACAGAAACAATAAATGTGTAACACAACGATGCAGCAAAACTTTAAATTAAATGCGCGACGCAGAATGGCCATTAAGGACTTTTAAGTGAGGAGCCGCAGAGTGGGCCAACATCAATCACGGAGACAACAAGCCGCTGGCACCCTTAAAGCCCCCCTTAAAGCCCCCT

 

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