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CRIMIC CR70385

Gene Acph-1

CG7899

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:29991144..29993411	99D1	FBti0226917	97311

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGTGTACTTTTAAGACCAACATCAAC	CAACTTCAAGATCGCTATTCACAGTC

Images

Construct Design Sequences

sgRNA sequence	CGAACAGATCGCCAGTTATACGG
sgRNA2 sequence	CACTTTGGCAACTAGAGAGCGGG

 

left_200bp	right_200bp
TTGCGTTTTTAAATCTGCCCGCCAAAAGATTACTGTTAGGGTCTAGGGTTGGTTGGGGACAACTATCGTTATCGAGACTTCGAAGCTTTGTGTGGTTATCAAACAGGTCCCCAAAATTATAAACAAAGAAATCAGAAATTTGGTAAACTTCAGACTGCCTGCTGTATATATGCGAACGACGAACGAACAGATCGCCAGTT	CTCTAGTTGCCAAAGTGATTTGCCTTTCATGGTGTTTTCTATATTGATTTTGGAAGTCATTGGAGTCTCAGGCGACAGTTGTTGTTATTCGTGGTTACTACTTAGTTAAGCCGTGCATTTCACACAAAGTCAGTTTGTATTGATTAGTTTTGTTAGTTTAAGTTTGTGCATTTGAAATTGAAAAACACAAGCTCGATAGC

 

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