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CRIMIC CR70387

Gene Spp

CG11840

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:542786..544653	21E2	FBti0226919	97313

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGGTGAACAATTAGCTCGATAAGC	CGCATTACTGTTTACACAAATTACTGC

Images

Construct Design Sequences

sgRNA sequence	TGAAAATCGCCGGCTTGCGGGGG
sgRNA2 sequence	GTGTTAGTCCATATGAGCTATGG

 

left_200bp	right_200bp
CAAATCAGATTTATCAGTTTTGAATCTCAACCGTTTTGGTATATTTTTCGGGAGTTTGGTGGCGGTGCGACCCCACCACAGAGGGTGGTATATTTTTGGTATATTGCGGTGCGTCCGATAGGCCCAGACGATGACCAGCGGTACCGATAACACAGATTGTTGACGTGTGCTGCTGCGTGTCATTTCGCTTTTCACCCCCG	CTCATATGGACTAACACCGCTACAAAAGACGATTCACTTACTGAGTTAGTTATGTACGTTCCTTGGCCAGGCACAACAATCTTATTTAGTTCGGTTAGGTTACAATTGTAGAAAATGTTCCCTCGACTTTTTTACCGCAATCAATTTAAATATGTATTTCAAATATTTCTGTGGCTCCGCACAGGAGGCTTATATGTTTA

 

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