CR70388

CRIMIC CR70388

Gene AP-2mu

CG7057

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:22462455..22464603	94A15-94A16

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GAGAGCTGTCTAGAAAAGAACTGATG	CTAGTAGTGGTATGTCTATGGGGAAC

Images

Construct Design Sequences

sgRNA sequence	CCTCGAAGGGCCACACTAAGGGG
sgRNA2 sequence	GCGCCCAAGCATCCCCATCACGG

left_200bp	right_200bp
TATTTAGTTGTAAAATAATTGTTGAATCTATTTTTTTCCAATAACACGACTTATATATTTTTTGAAAATATTCGAGCTAAATCCCAAGAAGTAAACTCAATCTGGGATTTGAAGTGCCCAGAACTCGAATAAACACTTCTTTTTAAATAATTGTAAGACCGTATCACTTATGGTATATACTGACCTCGAAGGGCCACACT	TCACGGAGCAAGATTATCACAATTGAATTTAACGAGATGAATGGAGAAAGTGTTACCGCATCTATTGGTATTTCCTCAATCAAATTGAACCAGCAGCTTATGTATTCCAAGATTAGTTCCAGCAAGTCCGGAGGACGCCCGAGTAGCGGATCCACCCCGGACACCCTATGCCGTATACCGCACACACTGCCTCCGCGAGC