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CRIMIC CR70390

Gene koi

CG44154

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:6811019..6818199	42D4	FBti0220963	95066

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTCAACAAGTTGGACTTTGG	GGTGGGAAAGTTTCATTGGGG

Images

Construct Design Sequences

sgRNA sequence	CGTGTGCCGTGACCTTGTACCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATGTTTTCATCGGATCGGTGCTGAGTTTGGACACGTGGTTGCTGCGATCCTCGGATGCGGAAAACAAGTCGAAAAAGCGCTTCCTCATATTTCTGCTGATTTTGTTGCCCTTGCTGCTGCTGTCGGGTGAGTAGGATTTAAAGCACCGTCCGATTTTTGATGTAAAGTAAAATATGTTAGAGCGTGTGCCGTGACCTTG	TACCGGTTCGAGAATTCATACTACATGCATATAGATCACCTCCAGTCGTTTTATTTATAAACTCGTCTGGTCGTTGGCAGTCGCGTGACGCCATCGATAATTTGTTAGTAAAATCCCGCGGATCAGATTTAAGTGGCCATGTGATGACATATTTTATTTTCCATTCGCCGTCTGCCAAGGTTAAACGCCTAATTTGCTTT

 

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