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CRIMIC CR70391

Gene CG3036

CG3036

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:4892112..4903889	25B1-25B2	FBti0226862	97163

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CATGGACTAAATTTCGAGTAAAATTTCGC	GCTTAAGCACCTTGAATTTTGTTTATACAAAG

Images

Construct Design Sequences

sgRNA sequence	CGTTTATAGCCTCTAATTAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGATGAAGTGGTGGCGGGTGGGTTGATCGCATGTTTGAGTGCACATTTCGTCGATCCCGTGAGCAATCATGAATGATAGTGAAGCGGCGAGCTCTAACCAAAGAGCAAATTGTTCAATTAGCAGGAAAATATTAAAAAATTGTAAGAAATACGTCTCAAGATTCCAAGATTCCGAAATGCACTCGTTTATAGCCTCTAAT	TAGGGGGAGTTGGTATTAAAACTCACTTTGAGTGTGCTAATTAGATACGTTGTCGCACGAGACAATTACAAATTCAATTAACACTAAAACAAACTGAAATTCATATCGATTAGACAGCTTATAGTATGGTTATTCATTACAAGCTCGTGTAATTCACACCCACTCAGTCACTCAGTCAATTGCAAATAATACAGGAAGGT

 

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