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CRIMIC CR70392

Gene Aph-1

CG2855

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:2766233..2767281	23A5	FBti0221010

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGCTCTGCAGTCTGGCAG	CCGCAAGTTCACCTTGACC

Images

Construct Design Sequences

sgRNA sequence	ATGACGTTGCCCGAGTTCTTTGG
sgRNA2 sequence	TTAACTAGGCGGTTAATACTAGG

 

left_200bp	right_200bp
AATTAAAGCAAAGGATAGGTGACTAAGCAATTTAGTTAAGATTAAAAGATTCCCATTTGTTTGGATAATTCTTGCAGTTTCAAAAATTTGTTTACTTTTGCGTGACGCACAAGCTAATGTAGTCATCTGTGTAGGGTATGTACACTACTAAAACGTACAGTGTGGCAGCCCGAATGGCACTGCGAAAGTCTCGCAACTCT	ATTAACCGCCTAGTTAATAGCTTTTGTATAACATTATAAAAAAAAACAAATTACCTTGTATATGCTCGATTAGTTACGCATTCTATGGACTTAAATCCCTTTACTAAATGGATTTCTTGAAAGAATGAGAATGCATTAAGATCCCAAGGTCTTTCGCAATAAATTCCCAAAACACAGATCTTAAGATACATTATTTTTAA

 

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