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CRIMIC CR70399

Gene Abi

CG9749

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:14119262..14122420	88A9	FBti0226863	97164

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGCTCGCCTATAGCTAC	CTTAAACTGGCGCGAGCTCAC

Images

Construct Design Sequences

sgRNA sequence	TGTCTCTAAGTACGACTCATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTCCTCGAGCTTCAGGCCCAGCAGCTCGGCGAGATGGAGTCCCAGATGAACCACATCGCCCAGACGGTGCATATCCACAAGGAGAAGGTGGCCAGGAGGTAAGAACAATCAATTGCTTAAACTAGAATAGGAAACGGGGATATGCATATTTTAAAACGACAAACAATACACAATGAAAATGTTTGTCTCTAAGTACGACT	CATCGGTGATAAAAGCTTCATAGAGAAGAAATGTACATTCATACATGCGTCGTCAGACGTGCTGATAAGACTGAACGTCTCTTTCTTCATTCTTTTCCTATATAACTATTTAGTTTTTTCAATTCACTTTATTTGTATTAATTATTTTTGATTATCTTCATTGTCTTTGCTCAGAGAGATTGGCGTGCTTACAGCTAACA

 

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